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In case no matching assays are found for the reference gene of choice (out of the listed assays), ProbeFinder SW suggests dual-color assays with one or more other reference genes out of the list.Designing multiplex assays is as easy as designing monoplex assays: when you select the multiplex option online, ProbeFinder will design multiple UPL assays for your gene of interest. Learn More. The John J. Wright Library houses a variety of instructional services & functions as an information center at La Roche University. This website contains information that is provided to a wide range of audiences and could contain product details or information which may not be accessible or valid in your country. Please be aware that we do not take any responsibility for accessing such information which may not comply with any legal process, regulation, registration or usage in the country of your origin.

Students performing research have access to reference materials in varied formats, as well as personal assistance from staff. The easy-to-use ProbeFinder Assay Design Software displays the target-specific primer sequences (ready for ordering at your preferred oligo supplier) and the matching Universal ProbeLibrary probe.Universal ProbeLibrary assays are compatible with all real-time PCR instruments capable of detecting fluorescein, FITC, FAM, and/or SYBRChoose the different Universal ProbeLibrary Products:UPL is based on only 165 short hydrolysis probes. More than 800,000 documents available on demand for Central Diagnostics, Tissue Diagnostics, Life Science, Sequencing and Customer Biotech product information. Robust and reproducible library construction in 2-3 hours with high conversion rates and library complexity, particularly for FFPE They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.The extensive transcript coverage of the UPL probes is due to their short length of just 8 - 9 nucleotides and the selected sequences. You will hear about upcoming events, and download the latest technical and scientific literature. Technical support: Local IT Service DeskA Media Library account is needed to access and download files in the Brand Centre. The locked ribose conformation enhances base stacking and backbone pre-organization, this gives rise to an increased thermal stability and discriminative power of duplexes. We're happy to offer a wide range of images and video footage relating to our people, medicines and diagnostic tools. To request an account, click "Create New Account" on the login page. Products are for life science research only. In order to maintain the specificity and melting temperature (Tm) that hybridizing qPCR probes require, The sequences of the 165 UPL probes have been carefully selected to detect 8- and 9-mer motifs that are very prevalent in the transcriptomes, ensuring optimal coverage of all transcripts in a given transcriptome.Within the human transcriptome, each probe binds to approximately 7,000 transcripts, while each transcript is detected by approximately 16 different probes. Barcode (found on the back of your school ID card) * Re-Type Your Barcode *. Manage your cobas e-library CDs preferences. In multiplex assays, a standard FAM or SYBRMultiplex PCR assays for a target gene and a UPL Reference Gene are designed by the ProbeFinder software at the Assay Design Center with a high success rate of over 90%. LNA nucleosides containing the six common nucleobases (T, C, G, A, U and mC) that appear in DNA and RNA are able to form base-pairs with their complementary nucleosides according to the standard Watson-Crick base pairing rules.The Figure shows the comparison of LNA, DNA, and RNA.Therefore, LNA nucleotides can be mixed with DNA or RNA bases in the oligonucleotide whenever desired. To request an account, click "Create New Account" on the login page. Please be aware that we do not take any responsibility for accessing such information which may not comply with any valid legal process, regulation, registration or usage in the country of your origin.Please complete the below form. Mannheim. Media library. Know what you can expect when you visit the library, including our recent service and space adaptations. Please be aware that we do not take any responsibility for accessing such information which may not comply with any legal process, regulation, registration or usage in the country of your origin.We use cookies to give you the best online experience.

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